Journal: Stem Cell Research & Therapy

Article Title: Apoptotic vesicles from endothelial cells promote endothelial progenitor cell differentiation and angiogenesis via miR-30a-5p mediated activation of the EGFR/PI3K/AKT/VEGF pathway

doi: 10.1186/s13287-026-04912-x

Figure Lengend Snippet: Differentiation and characterization of EPCs and ECs. A Schema for differentiation of hESCs to EPCs and then to ECs according to a reported method. B Immunofluorescence staining of hESCs markers: TRA-1-60, NANOG, OCT4 and SOX2. C Phase contrast showing the morphology of EPCs. D Flow cytometry analysis of surface markers of EPCs. E Phase contrast showing the morphology of ECs. F , G Relative mRNA expression of endothelial transcripts (CD144, CD105, and KDR) and markers related to hESCs (OCT4, SOX2, NANOG) of hESCs and ECs. H Immunofluorescent micrographs of ECs showing the expression of CD31, vWF and VE-Cadherin (VE-CAD). I Flow cytometry analysis of surface markers of ECs. N = 3 per group. * p < 0.05, ** p < 0.01

Article Snippet: Endothelial serum-free medium (ESFM), Human plasma fibronectin, DMEM/F12, and PBS were purchased from Gibco, Matrigel were purchased from Corning, SOX2, OCT4, NANOG, TRA-1-60, vWF, VE-Cad antibody was purchased form Abcam, CD31, CD34, CD105 antibody were purchased form elabscience.

Techniques: Immunofluorescence, Staining, Flow Cytometry, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Apoptotic vesicles from endothelial cells promote endothelial progenitor cell differentiation and angiogenesis via miR-30a-5p mediated activation of the EGFR/PI3K/AKT/VEGF pathway

doi: 10.1186/s13287-026-04912-x

Figure Lengend Snippet: Apo-EVs promote the differentiation of EPCs into ECs in vitro. A Images of EPCs under an optical microscope after different treatments. B – D Relative mRNA expression of CD144, CD105 and KDR of EPCs after different treatments. E , F Percentage of cells co-expressing CD34 and CD105 measured by flow cytometry after different treatments. G , H Tube formation of EPCs after different treatments. Scale bars: 200 μm. N = 3 per group. ns p > 0.05, ** p < 0.01, and **** p < 0.0001

Article Snippet: Endothelial serum-free medium (ESFM), Human plasma fibronectin, DMEM/F12, and PBS were purchased from Gibco, Matrigel were purchased from Corning, SOX2, OCT4, NANOG, TRA-1-60, vWF, VE-Cad antibody was purchased form Abcam, CD31, CD34, CD105 antibody were purchased form elabscience.

Techniques: In Vitro, Microscopy, Expressing, Flow Cytometry

Journal: Stem Cell Research & Therapy

Article Title: Apoptotic vesicles from endothelial cells promote endothelial progenitor cell differentiation and angiogenesis via miR-30a-5p mediated activation of the EGFR/PI3K/AKT/VEGF pathway

doi: 10.1186/s13287-026-04912-x

Figure Lengend Snippet: Apo-EVs Promote the Differentiation of EPCs into ECs by Activating the EGFR/PI3K/AKT/VEGF Signaling Pathway. A Volcano plot illustrates the DEGs between EPCs treated with Apo-EVs and the untreated EPCs. B GO analysis of DEGs. C Heat map of DEGs in the GO analysis related to cell differentiation, migration, and angiogenesis. D , E GSEA analysis and KEGG pathway of DEGs. F Expression levels of β-actin, VEGF, AKT, p-AKT, PI3K, p-PI3K and EGFR protein in EPCs after different treatments. G – I Relative mRNA expression of CD144, CD105 and KDR of EPCs after different treatments. J – K Percentage of cells co-expressing CD34 and CD105 measured by flow cytometry after different treatments. N = 3 per group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001

Article Snippet: Endothelial serum-free medium (ESFM), Human plasma fibronectin, DMEM/F12, and PBS were purchased from Gibco, Matrigel were purchased from Corning, SOX2, OCT4, NANOG, TRA-1-60, vWF, VE-Cad antibody was purchased form Abcam, CD31, CD34, CD105 antibody were purchased form elabscience.

Techniques: Cell Differentiation, Migration, Expressing, Flow Cytometry

Journal: Stem Cell Research & Therapy

Article Title: Apoptotic vesicles from endothelial cells promote endothelial progenitor cell differentiation and angiogenesis via miR-30a-5p mediated activation of the EGFR/PI3K/AKT/VEGF pathway

doi: 10.1186/s13287-026-04912-x

Figure Lengend Snippet: Apo-EVs promote the differentiation of EPCs via miR-30a-5p. A Top 20 miRNAs were detected in Apo-EVs, and after comparing with the 1042 miRNAs capable of targeting PML, 6 miRNAs were screened. B miR-30a-5p showed the highest expression level among the 6 screened miRNAs in Apo-EVs. C , D . Percentage of cells co-expressing CD34 and CD105 measured by flow cytometry after different treatments. E , F Relative mRNA expression of CD144 and CD105 of EPCs after different treatments. G Expression levels of PML, β-actin, VEGF, AKT, p-AKT, PI3K, p-PI3K and EGFR protein in EPCs after different treatments. N = 3 per group. * p < 0.05, *** p < 0.001, and **** p < 0.0001

Article Snippet: Endothelial serum-free medium (ESFM), Human plasma fibronectin, DMEM/F12, and PBS were purchased from Gibco, Matrigel were purchased from Corning, SOX2, OCT4, NANOG, TRA-1-60, vWF, VE-Cad antibody was purchased form Abcam, CD31, CD34, CD105 antibody were purchased form elabscience.

Techniques: Expressing, Flow Cytometry